Cv. the Azine and Azonium Compounds of the Proteolytic Enzymes
نویسنده
چکیده
THE immense importance of the establishment of the chemical nature of the proteolytic enzymes has led to many endeavours to purify them. From the earliest attempts of Schwann [1836] to the present day all these have met with obvious failure. In 1905 it was observed by T. Brailsford Robertson [1907] that if one drop of saturated safranine (Griibler) is added to a solution of trypsin (Griibler) a precipitate forms which flocculates in the course of a few hours and collects at the bottom of the tube. Some years later Holzberg [1913] demonstrated that the precipitate was proteolytically active. With these two facts in view a study of the nature of this precipitate was undertaken.
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